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Jackson Laboratory chat ires cre mice
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Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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Jackson Laboratory chat ires cre ∆neo transgenic mice
Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) in ChAT‐Cre mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.

Journal: CNS Neuroscience & Therapeutics

Article Title: Whole‐Brain Neural Connectivity to Cholinergic Neurons in the Lower Thoracic Intermediolateral Column

doi: 10.1002/cns.70902

Figure Lengend Snippet: Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) in ChAT‐Cre mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.

Article Snippet: Five adult ChAT‐Cre mice (male and female, RRID: IMSR_JAX: 018957, C57BL/6J background strain, 2–4 months, 25–27 g) and three wild‐type littermate mice were used in experiments.

Techniques: Labeling, Fluorescence